Marine Algae Incorporated Polylactide Acid Patch: Novel Candidate for Targeting Osteosarcoma Cells without Impairing the Osteoblastic Proliferation

Biodegradable collagen-based materials have been preferred as scaffolds and grafts for diverse clinical applications in density orthopedy. Besides the advantages of using such bio-originated materials, use collagen matrices increases risk infection transmission through cells or tissues graft/scaffold. In addition, solutions are not counted economically feasible approaches due to their high production cost. recent years, incorporation marine algae synthetic polymers has considered an alternative method preparation grafts/scaffolds since they represent abundant cheap source potential biopolymers. Current work aims propose a novel composite patch prepared by blending Sargassum vulgare powders (SVP) polylactide (PLA) porcine-derived membranes. SVP-PLA patches were produced modified solvent casting method. Following detailed material characterization assess cytocompatibility, human osteoblasts (HOBs) osteosarcoma (SaOS-2) seeded on neat PLA patches. MTT BrdU assays indicated greater cytocompatibility higher proliferation HOBs cultured than those PLA. SaOS-2 exhibited significant decrease cell proliferation. The described herein exhibits antiproliferative effect against without impairing HOBs’ adhesion